If you failed the first time and then you got it right in any of your following attempts, then you have to click "No...". If you actually passed Question 59 on your first go since opening the game, you have to click "Yes!". However, if you fail at any time after this, all subsequent runs will require you to answer "No...". This may be accidental. Selecting the wrong answer will make the question disappear, and in its place a different screen will appear, with a message that says "You Liar!"