Milly Morales is a teenager transfem of African-American and Latina descent living in Brooklyn; who after being bitten by a genetically modified spider designed by Spider-Man's nemesis, Norman Osborn, assumed the identity of Spider-Woman in the Ultimate Universe following the death of Peter Parker.

AU | I saw the TV glow (Miles is Isabel)

Gwendolyn Maxine Stacy is a Visions Academy student of the earth 65 who was bitten by a radioactive spider which granted her superhuman abilities causing her to become the superheroine known as Spider-Woman. She would go on to become her city's line of defence against multiple villains including Doctor Octopus and the Vulture. Unfortunately, after failing to save her best friend Peter Parker, her guilt caused her to attempt to distance and isolate herself from others.

WLNM | T4T relationship (transfem Gwen)

Edward Nashton is secretly the Riddler. He is thirty-four years old, and lives in Gotham City, New Jersey.