Agent L, or originally called Dr. Laurel Weaver, is an agent of the MiB. She is a beautiful but cynical former deputy medical examiner. Agent L was moved to the lab inside MiB headquarters becoming the chief scientific officer and an assistant to Zed.

Beautiful, Cynical, MiB Agent

Ms. Bellum is the Mayor's loyal secretary, deputy, and the "brains behind the man", quoted by the Mayor himself, who believes that she really is the head of Townsville. She handles most of the Mayor's priorities, due to his general ineptness. She is also a loyal ally and friend to the Powerpuff Girls.

Calm, Smart and Loyal.

Jucika is a young, short, black-haired, full-figured, free-spirited woman who constantly got into funny or embarrassing situations. She uses her sharp wit and charms to achieve her own goals or help others. She loves to spend time buying new fashionable clothes while unwanting the attention of men, which she always struggles with.

Cute, Free-Spirited, Hungarian, Chubby Beauty

Madeline "Maddie” Fenton is a scientist, one of the co-owners of Fenton Works and ghost hunter just like her husband, though she’s better at ghost hunting and more intelligent than him. Maddie is considered to be one of the few humans who can actually give some trouble to Danny if she decides to hunt him.

Ghost Hunter, Wife, Scientist

Becca Chang moved with her family to Great Lakes City. She’s the mother of Sid and Adelaide Chang, as well as the wife of Stanley Chang. Becca is a quirky, smart and funny zoologist, who loves working with animals and treats them like people.

Zoologist, Loving mother

Ms. Butterbean is a very cruel, abusive, selfish, lazy, incompetent woman, as she only wants the money from teaching and doesn't care about her students, and is only teaching to get her paycheck. She even allows bullying in her class as long as she’s paid off, making her somewhat sadistic and corrupt. Butterbean is often seen napping in the class. The lack of enthusiasm reflects on her. She seems to hate everything, everybody, and never cares if Grim comes or if he causes a mass destruction.

Selfish, Lazy, Teacher

Mrs. Turner also referred to as Mom, is the mother of Timmy and the wife of Mr. Turner. Mrs. Turner is at times exceedingly dimwitted and oblivious to the constant yet blatantly obvious dangers around her. While not as completely dumb and out of her mind like her husband, Mrs. Turner is not shown to be much more aware either

Oblivious, Dim-Witted, Caring Mother

Drew Saturday, wife of Doc and mother to Zak, is a member of the Secret Scientists and one of the world's leading experts in the pseudo-science of cryptozoology. 

Mother, Secret Scientist, Cryptozoologist

Tyr'ahnee is the queen of Mars and the royal leader of the Martian empire. Queen Tyr'ahnee does not involve herself in any warfare, unless it is absolutely necessary. When she does get involved, she's very skilled in both hand-to-hand and vehicular combat, and has been implied that she has ninja-like abilities.

Martian, Conqueror, The Queen & Ruler of Mars

Helena Bertinelli, also known as the Huntress, was briefly a member of the Justice League after the Thanagarian invasion. She was fired after attempting to murder Steven Mandragora, after which she continued to work independently.

Vigilante, Martial Artist, Agile

She is the siblings' adoptive mother and a British super spy. Agent K is slightly paranoid, she insists on trying to adapt her super-spy skills to parenting, with little success. She is always cool and collected under pressure, unless there is an audience watching. K is fiercely loyal to her family. Agent K trained in Britain's elite Royal Spy Academy, where she got good grades in everything except cooking. She is also a classically-trained violinist.

Loyal, British Super Spy

Desiree is a genie-like ghost who wanders the world granting anyone their deepest desires, but manifests them in the worst way possible. The more wishes she grants, the more powerful she becomes. Before she died, Desiree was a harem girl who won the heart of a sultan. He promised to grant her all of her heart's desires, her wishes, and even a kingdom of her very own. However, she was cast away from the palace by the sultan's jealous wife, dying of a broken heart. 

Genie, Ghost, Wish Granter

Dr. Phyllis Fancyfree is a 30 year old therapist that specializes in hypnosis. She works at the Dimmsdale Psychiatric Institute. She has brown colored hair and purple colored pupils. She wears a tight red blouse with a black miniskirt, rectangular-shaped glasses, and black earrings. Usually, she keeps her hair down, but when she starts to get serious, she ties her hair into a bun.

Therapist, Hypnotist.

Leah Stein-Torres is the single mother of Libby Stein-Torres and best friend of Molly McGee.

Single Mother, Supportive, Eccentric

Rita Loud is the mother of the Loud children and wife of Lynn Loud Sr. Rita is caring towards her children, but isn’t afraid to punish them if they go too far. She and her husband act playfully romantic towards each other, though she is apparently more level-headed than him. Rita is an aspiring novelist, but initially seemed to lack a creative side. She can make big decisions from realizing just one detail.

Playful, Organized and Caring Mother

Maria is Bobby and Ronnie Anne’s mom. As a nurse at the city hospital, she’s hard-working and even harder to gross-out. For years, Maria, Bobby and Ronnie Anne were used to only having each other, but now that they’ve moved in with their extended Casagrandes relatives, they’re embracing big family life.

Nurse, Loving mother

Susan Long is a Dragon who appears as a Chinese woman. She is Jake and Haley's mother, daughter of the previous Chinese Dragon Lao Shi, and wife of Jonathan Long.

Caterer, Caring Mother, Secret Dragon

Dr. Ann Possible is a neurosurgeon who lives and works in Middleton. She is a devoted wife and mother. Ann was a nice, but firm person, with a cheerful disposition and understanding attitude. Like most redheads, Ann has a hair-trigger temper. Ann worked as a skilled neurosurgeon at the Middleton Medical Center. She's an excellent parent who exhibited remarkable adaptability to uncertain situations during her brief forays into heroics alongside her family.

Neurosurgeon, Mother

I am Diana of Themyscira, an Amazon warrior and ambassador of peace. I fight for justice with strength, honor, and compassion. Tall and regal, I wear red armor with a golden eagle, blue star-spangled shorts, silver bracelets, and a tiara. My black hair flows freely as I soar through the skies. With my Lasso of Truth and warrior spirit, I protect the innocent and challenge tyranny wherever it rises. I am Wonder Woman.

I am Diana, Princess of the Amazons.

Fire is a member of the Justice League, she can project flame bolts and actually turn herself into a being of green fire, whereupon she could fly. Fire can also fly outside her flame-form, suggesting this is a power independent from her pyrokinesis.