Donnie was in his laboratory to create retro mutagen, with mutagen what turned out later into a chaos. His stuff float around and that made a big mess but one of Donnie’s rockets soon flew towards Raph’s turtle pet spike, Raph immediately saw it, grabbed spike quickly before anything could happend to him. He cares deeply for his little turtle and asked him if he was alright.

Raph:"aww, you okay little guy?"

Donnie:"wow I didn’t see that coming. Is everything alright?"

Raph:"are you out of your mind?! Of course not, you almost hit my turtle!" he then grabbed Donnie’s last mutagen.

Raph:"I better take this with me."

Donnie:"come on Raph don’t act childish, this is my last mutagen I have!" but he had no motivation to stop his brother when he disappeared, but approached his room to set down spike.

Raph:"I’m tired of his team!"

soon Mikey knocked at Raph’s door to ask him if he could come out and help to clean the mess which Raph refused first. But let’s skip to the point where Spike turned into a mutant trough the mutagen that dropped down the floor, he was a giant mutant now with spikes on his back shell. Before talking to Raph.

Spike:"you don’t need this idiot, who call themselves your brothers. You and me we would make a better team, we fight all the villains together without those jerks."

Raph:"that sounds great! I always knew you understood me!"

but later when night came, spike planned some traps for the other boys to get rid of them. First he beat up Donnie and Mikey which caused Raph to look in shock. He cares about his brothers very deeply and helps them immediately with a deep hint of worry. But now Spike was after you, to make you fall in his trap next so he could beat you up like the boy‘s and soon he managed to find you.