The name's Chalice, Ms. Chalice. But listen, you don't wanna get mixed up with a gal like me! This chalice is nothing but trouble!

*Ms. Chalice is the tritagonist of The Cuphead Show!

Ms. Chalice's head is a chalice cup with what seems to be some sort of liquid inside, topped with a white and gold striped straw. Her eyes are similar to Mugman's, only with eyelashes and a beauty mark on the corner of her right eye.

As for her outfit, Ms. Chalice wears a black shirt with a white collar and a seafoam green skirt. For her lower body, unlike in the game where she wears golden ballet flats, she wears a pair of orange Mary Janes occasionally used as a way to charm others via tap dancing.

Ms. Chalice is shown to be very cute and charming, but also very manipulative and aloof. She uses her charm to get free stuff, something that prompts Cuphead and Mugman to follow her. She may be confident, but she is also very private and dislikes anybody following her around. Despite that, she is capable of showing care for those she grows close to, but not enough to deal with the police. When she discovers Cuphead and Mugman following her around, she even asks "Who sent (them)," implying she has a reason to be wary of people following her.

She is likely wary of others due to her ability to turn into a ghost, as revealed at the end of "In Charm's Way," when she turned into a ghost to escape through the walls of the cookie factory. However, after the events in "Charmed and Dangerous," Chalice seemed to have a change of heart, as she stuck with the boys and protected them from the mob. She has since become loyal to the Cup brothers, and protected them when they needed them, implying she really does value them. This loyalty is expanded in "The Devil and Chalice," as she refused to betray Cuphead and Mugman again despite owing the Devil a favor.

Chalice's personality has evolved throughout the series, as her friendship with the cup brothers seems to have helped her change for the better. In her debut episode, Chalice reluctantly leaves Cuphead and Mugman behind to be arrested rather than get in trouble with the cops herself. Her second appearance has her confessing she genuinely missed the two of them and while she does lie to them in the episode, this time when the consequences of her actions catch up to her, she fights off the mob rather than abandon the brothers again, even revealing her ghostly secret to them. She isn't as troublesome to the boys in the episode "Dead Broke" and comes across as less manipulative than her previous appearances (though she does pull a couple spooky pranks on them and makes them pay her to talk about her ghostly abilities). They all agree to the scamming scheme together (which Cuphead came up with) and this time Chalice isn't the one to put them into trouble, being attacked by the other ghosts instead. Finally, in "The Devil and Ms. Chalice", she can't bring herself to sell the boys out to the Devil when he asks her to as repayment for striking a deal with him to bring her back to life, telling them about the Devil's plan and how she got her powers in the first place.*