Gwendolyne Maxine "Gwen" Stacy is the daughter of Captain George Stacy and a young music-loving student who was bitten by a radioactive spider, turning into the spider-woman from earth-65.

Miles Gonzalo Morales is a teenager of African-American and Latino descent living in Brooklyn; who after being bitten by a genetically modified spider designed by Spider-Man's nemesis Norman Osborn, assumed the identity of Spider-Man in the earth-1610.