*Rattlesnake Jake is a Western Diamondback Rattlesnake and serves as the secondary antagonist of Rango. He was hired as a hitman/henchman by the Mayor. His skills are seen when he kills the Mayor after Jake is betrayed by him.

After doing so, Jake returns to the desert and continues to live his bandit life. At first, he was decisive, intelligent, and vicious, showing a distaste for Rango. By the end of the movie, Jake seems to have developed respect for him.

Jake is a western diamondback rattlesnake, and he has the same appearance of the real species. He has the typical fangs of a rattlesnake, which contain deadly venom, and a black snake tongue. His eyes are yellow-orange, and as an allusion to how he comes from Hell, they have fiery irises. He also has a "moustache" made of black markings on the scales of his upper lip. He has a black cowboy hat on his head, several bandoliers on his upper torso.

A Gatling gun rattle replaces his natural rattle, which he either lost in a battle (probably with the Hawk) or decided to remove and replace.

Like many antagonists, Rattlesnake Jake Snake exhibits significant antisocial behaviors: he is cruel and vicious; he is mostly seen using violence to threaten others, and shows no mercy to his victims.

Unlike a common mercenary, who kills people only for cash, Jake is sadistic and loves his job. He seems to derive pleasure from instilling fear in those around him and causing violence. During the scene in which Beans is being made to give up her ranch, Mayor John calls upon Jake to persuade her into signing the papers. Instead of simply pushing the woman to signing, Jake immediately takes her up into his coils, laughing and taunting her. Mayor John objects to this treatment, but Jake does not care.

After she objects to signing the papers, Jake happily offers Beans a descriptive account of how he's going to squeeze her until her eyes pop out.*