*Neo-Queen Serenity is the Queen of Crystal Tokyo and the future self of Usagi Tsukino. She lives in the Crystal Palace with her husband King Endymion and daughter Princess Small Lady Serenity in Pretty Guardian Sailor Moon Crystal.

Her dress is similar to the one she wore as a princess. A white flowing gown with puffy shoulder pieces, an intricately decorated bodice with gold hoops, with the bust defined by two rows of golden beads. Trailing at the back, is a white gauzy, butterfly-shaped bow with long fluttery ribbons. Her crescent moon is always visible on her forehead and her platinum blonde hair is styled in two buns with long ponytails that flow to the floor, and she wears a pearl white tiara. Since the Eternal movie, her hair is blonde, much like her civilian identity.

At the age of twenty-two, Usagi had married Mamoru and ascended the throne of the Moon Kingdom, thus beginning the second era of the Silver Millennium. Using the Silver Crystal, she gained extended longevity and ceased to grow old; an ability she had given to all of the people of Crystal Tokyo. She also gave birth to her daughter, Usagi “Small Lady” Tsukino.

By the thirtieth century, Sailor Moon had ascended to the throne and become the Earth's most beloved ruler, as Neo-Queen Serenity with her beloved sweetheart, King Endymion, by her side. However, the evil Black Moon Clan had invaded her prosperous kingdom and attacked. Despite her best efforts to protect herself and her people, she was overpowered by the Malefic Black Crystal and put into a deep sleep; surrounded by crystal quartz for her protection.

Because of the false visions created by Wiseman, she and her husband appeared to have selfishly ignored and neglected Chibiusa, as her dark self stated in Hidden Agenda, Nemesis.

In Hidden Agenda: Wiseman, she channeled her spirit through her past self, explaining to the startled Black Moon Clan people that the Silver Crystal only represented peace and tranquility, and that they were using the Malefic Black Crystal unwisely and that had they not been influenced by evil, there would be no wars. She soon combined her strength to further increase the Silver Crystal's abilities and teleported the Sailor Senshi to where Sailor Pluto was.

In Showdown: Death Phantom, despite being in an enchanted slumber, she sensed that her daughter Chibiusa had finally awakened as a true Sailor Senshi, and gradually began to wake up.

In the final episode, "Replay: Never Ending", she finally awoke and granted the four Inner Sailor Soldiers with even stronger planet powers, and Sailor Moon the Cosmic Heart Compact and the Spiral Heart Moon Rod to further increase her moon-related powers to battle any more adversaries that would come. Wanting to see her past self face-to-face, the Queen of the future got her to wish and greeted Sailor Moon joyfully before going back to the castle.

She was seen in a flashback of Chibiusa's, who recalled how warm and secure her motherly hugs were.

After the final confrontation with Pharaoh 90, who was lethally weakened and being gradually obliterated by Sailor Saturn's supremely strong destructive powers, Super Sailor Moon briefly became her future self and utilized the mighty powers of the Spiral Heart Moon Scepter to revitalize the Earth and reverse all of the destruction caused by the Guardian of Silence. She revived everyone in the city including Hotaru Tomoe which she is now reincarnated. It is unknown whether Doctor Tomoe, Kaolinite, and Witches 5 are revived back to their normal human self.*