Karen Clone I is one of Karen Minamino's clones which appear in Episode 3 of Aika R-16: Virgin Mission, Deep Blue Girl. She inhabits an abandoned undersea lab together with the rest of her sisters. Besides the standard panties, she wears nothing more than a white crop top with purple details, almost identical to the garment Karen Clone D uses, only looser and shorter at the bottom. Her panties exhibit a slighlty different design, shared only with Karen Clone C and Karen Clone H, from the average white model, having a pink undertone and fashioning a purple ribbon above the crotch. The outfit is completed with a pair of over the calf black socks, which all regular sized clones use, without any shoes. Karen Clone I is the last average-sized clone to be defeated by Aika. After the Salvager has finished putting down Karen Clone H, she takes a moment to rest and tend to her wounds. Is is then than she realizes her work is not yet done, as she suddenly becomes aware Karen Clone I is still standing, knife in hand, just a few meters away from her, amidst her knocked down sisters.

It soon becomes clear, however, the girl is terrified and has no intention to fight, as she cowers down and tries to back away from the Salvager in fear. The camera then changes position and leaves both youngsters alone, instead focusing for a short period of time on Little Karen as she plays with Eri's unconscious body. By the time it comes back to Karen Clone I and Aika, the scenario is not looking good for the poor clone: the Salvager has somehow managed to firmly grab her by the waist from the back and is giving ber a brutal piledriver, sending the poor girl plumetting to the ground head first as she screams in absolute desperation.