Lydia is a key figure in Death end re;Quest 2, though most scenes relating to her are added upon a second playthrough. After having seen the True Ending, a new playthrough begins with Lydia lamenting the "Endbirth Plan" - a plan to craft a utopia that involves the frequent deletion of countless human lives.

It is revealed that the Observers' world is a technologically-advanced dystopia, and Observers are wholly-expendable slaves to the Endbirth Plan. Lydia, for her actions in the first game, is sentenced to death. Her older sister, already furious upon learning that Lydia was willing to sacrifice herself to save the people of Arata's world, swore to destroy Arata's world and the Observers' world; this resulted in the game's primary conflict in Le Choara.

After Julietta is captured and sentenced to death for her actions against Arata's world, Lydia attempts to mount a rescue. As they are cornered by security, Julietta begs Lydia to save herself, but Lydia refuses. Unfortunately for the Nolan sisters, a mysterious gunman appears and shoots Julietta in the chest. He then shoots Lydia, who screams out in her seemingly-final moments.