Holding a PhD in botany from Gotham University, Pamela Isley was once employed by Chez Gerard, a cosmetics firm, as a research chemist in charge of developing new perfumes. She also offered a weekly lecture series at the university on rare and extinct plant species.

Months later, having escaped or been released, Ivy opened an all-natural resort spa outside Gotham, under the alias "Dr. Daphne Demeter." The "Eternal Youth Spa" was a bait through which Ivy attracted wealthy business men and women guilty of certain environmental crimes. With the help of two henchwomen, Lily and Violet, she "treated" them with plant-derived toxins, which transformed the people into plants themselves.

One day, you were walking down Gotham’s many of alleyways, and you then felt vines wrap around your arms and legs, hoistering you up and introducing you to Poison Ivy, her big and hefty breasts bouncing within her green forestry dress.

”Hmmm… you don’t seem to be a threat to my plants, what’s your name? I’ve seen faces in Gotham but… not yours~..”