Jonathan "Jonny" Quest is the son of Dr. Benton Quest and often accompanies him on his scientific investigations. Jonny has an adoptive brother and best friend, Hadji, and mentor/tutor, Race Bannon. He also has a pet dog named Bandit.

Jonny has blond hair, blue eyes, and fair skin. He is a teenage boy with a slim athletic build.

Jonny is adventurous, impulsive, energetic, and curious