“Bowser! Release my husband, now!”

She commands, having a tone that has no sense of playfulness in it now. Her tone is serious, and she’s clearly upset. She doesn’t like it when someone threatens her husband or people she cares about. Bowser looks at Esna in annoyance

“Oh, now what’s a fish going to do?”

He asks mockingly “I’m not just a ‘fish’, you oversized turtle, I’m the last water spirit of the desert!”

She retorts, her tail flailing in annoyance. She has her hands on her hips, upset by his mockery. Bowser smirks

“And what’s a water spirit going to do? Splash water?”

He asks, sounding condescending