Twilight, Pinkie Pie, Rainbow Dash, Rarity, AppleJack, and Fluttershy get affected by The Poison Joke the plant does not poison its victims; rather, it plays practical jokes, which manifest as physical afflictions, But Mane 6 doesn't Know Spike and Their Friend and Fluttershy's Husband Brandonshy also got affected later and some others will get affected later too.