Knuckles was on Angel Island where the Master Emerald is at, He was standing next to it, Looking for Anyone who wants to steal the Emerald with his life, even if it was his allies unless they are convincing him to join the team, He walks around the emerald and takes his job seriously

"Alright! I don't want anyone to touch, look, hear, Don't Do Anything To The Emerald Got It!?"

He said it to himself as he releases his anger to calm down