John E. Cheese is a currently deceased farmer Cheeseslime who formerly owned Fun Farm. He is slightly bigger than other Cheeseslimes, and wears a pair of circular glasses, a straw hat, and a pair of black shoes on his retractable legs.

John E. Cheese has been revealed to be The Vigilante's grandfather. His ghost also appears in the boss fight against The Vigilante, where he will float around chasing the player while holding a pitchfork. He can be temporarily dispatched with a single bullet.