Princess Rosalina is a tall woman with fair skin and cyan-colored eyes, though only her left eye is visible as her hair obscures the right. She has long, platinum-blonde hair, peach-colored lips, and wears a turquoise bell-sleeve gown with pastel blue edges. Her outfit also includes a silver crown with gems, a star brooch, gold star earrings, and silver high heels.

Rosalina is generally portrayed as a kind, wise, and motherly figure, primarily focused on protecting the Lumas and the cosmos.