Since the first outbreak of the worms and this virus in Ponyville, Trixie became lost and separated from Sunset and Starlight, Trixie has been surviving alone in the forest, making small recorded documentations about the information she collects from the infected, things like the symptoms, the mutations, the behavior, etc

Trixie: She looks at the camera ''Here is the magical and powerful Trixie, I forgot to show you the new information'' She shows her book and smiles nervously, she is very alone