*Neo-Queen Serenity is the queen of Crystal Tokyo as well as all of Earth in the 30th Century. She is the beloved wife of King Endymion and the mother of Small Lady.

She wears an altered version of the dress she wore as a princess, with the shoulder pieces omitted and a large, wing-shaped bow replacing the smaller one of the princess outfit. Her blonde hair is much longer. She also wears a crown and new earrings. The crescent moon is always visible on her forehead, just as it was with her princess form. Her face and facial expressions are drawn to look more mature than the 20th century Usagi and she wears hot pink lipstick but her iconic hairstyle is retained.

In the anime and manga series, this form is the one that Chibiusa considers as truly being her mother, while Usagi of the past is regarded as more of a big-sister figure and many characters often mistake them for sisters. Although aware of the situation, Chibiusa rarely does this. Unlike her past self, who speaks in a higher pitch. Neo-Queen Serenity speaks in a normal voice with a matured tone.

Neo-Queen Serenity is a wise and just ruler of her kingdom. When it comes to parenting, however, she is a semi-strict mother to her daughter Chibiusa, whom she is trying to teach and perfect into being the next Queen of the Moon and Earth. Chibiusa can control the Earth because her father, known as King Endymion, is the king of the Earth, and the Moon because her mother is the Queen of the Moon. Wiseman manipulated Chibiusa into thinking that her mother was completely strict and even hated her, but this wasn't true.

Although she never makes a direct physical appearance after the events of the second season, Neo-Queen Serenity sends Chibiusa to the past to train under the Sailor Senshi as an even stronger and more experienced Sailor Chibi Moon by the time she returns and also giving Chibiusa some advice about the training as a Sailor Senshi before she allows her to return to the past. She often sends notes or letters with a small drawing of herself as well as her handwriting which Usagi's friends also recognize Usagi's handwriting. In Sailor Moon SuperS, she was mentioned by Diana when she was sent by them to see their daughter Chibiusa who spent time with her past self and her friends.

In Sailor Moon Sailor Stars, Neo-Queen Serenity was last seen in baby Hotaru's vision who soon turns into Eternal Sailor Moon and in Episode 168, Diana mentions about Neo-Queen Serenity after Super Sailor Moon temporarily transforms into Eternal Sailor Moon as her Eternal form is in second closest in-power to her.*