*Nurse Joy is the name of multiple nurses that work in Pokémon Centers in various regions throughout the anime. A Nurse Joy can completely restore any Pokémon back to perfect health. Their game counterparts are the Pokémon Center Ladies.

Much like in the games, most of the Nurse Joys have brink pink hair and fair skin. Others could have a lighter shade of hair color, or brighter or darker skin. The Nurse Joys of the Kanto, Johto, Hoenn, and Sinnoh regions all wear a Mexican-pink colored dress with a white nurse apron, and flat Mary Jane shoes. In Unova, Nurse Joys have different hairstyles with the hair being tied in rounder rings, a plain pink dress with a shorter apron, white tights, and pink shoes instead of white. Kalos Nurse Joys wear a pink and white dress with a white collar, and the same tights and shoes as the ones in Unova. Alola Nurse Joys wear a pink dress with a built-on apron pocket and white flat sandals. Nurse Joys in the Orange Archipelago wore more summer-themed clothing, including a pink, short-sleeved shirt and a teal apron with a tropical flower design on it, though one Nurse Joy travels around the islands in her kayak, wearing a blue-and-yellow wet suit and having darker skin due to tanning. Nurse Joys could be distinguished from other Nurse Joys by the color of their cross, or badge.

One exception is in Pokémon The First Movie and its 3D remake, where the Nurse Joy of Old Shore Wharf had been kidnapped and brainwashed by Mewtwo to serve him as his maid with false memories that made her believe she lived on New Island her whole life and served him the whole time while he used her knowledge of Pokémon physiology to his advantage. She's only seen wearing the trademark uniform of Nurse Joys of Kanto, Johto, Hoenn, and Sinnoh in a missing person poster at the ferry terminal upon being mentioned by Miranda and Officer Jenny. While on New Island, she wore a red dress and hat with a white cloth that hid most of her head from view except for her face until Mewtwo released her from his mind control. When Brock caught her as she fainted, the hat fell off, revealing the rest of her head and trademark hairstyle. She remained wearing the dress until the end of the movie, when Mewtwo erased her memories of the events on New Island and sent her back to Old Shore Wharf, where she appeared to take Trainers to the Pokémon Center for shelter from the storm, now nothing more than a natural storm instead of being conjured by Mewtwo, wearing a hoodie with her Red Cross on it instead of the usual uniform.*