*Gwendolyn Stacy is friends with Peter Parker and Harry Osborn. She is the daughter of New York City Police Department captain George Stacy. Despite romantic feelings between her and Peter, she dated Harry when he began dating Liz Allan.

Gwen was smart, but, a little nerdy. She definitely wasn't unattractive, but, compared to other girls such as Mary Jane, Sally or Liz, she was often overlooked by boys, with the notable exception of Peter and Harry. She was a hard worker, especially while being an intern at Curt Connors' lab. She is very attractive and beautiful when she was nerdy later on. She was the intellectual counterpart of Peter, rivaling his already vast intelligence.

After she was given a makeover by Mary Jane, she noticeably did not change her personality at all. Still being friends with Peter, she noticeably did not talk to him as much, possibly because she did not want Harry to think of her as being interested in him.

Gwen is voiced by Lacey Chabert.

First regular appearance of Gwen Stacy. She previously appeared in the series finale of Spider-Man but only as a cameo in an alternate universe.

Her appearance and personality seem to be closer to that of the comics character of Debra Whitman. Her appearance change towards the end of the series more closely resembles the Gwen of the comics though she retains her nerdy personality.*