*Daphne Blake is a "mystery hunter" and a member of the Scooby Gang, along with Scooby-Doo, Shaggy Rogers, Velma Dinkley, and her boyfriend, Fred Jones on the TV show Scooby-Doo, Where Are You! as well as multiple other TV and movie variations. She then appeared in the official Scooby-Doo, Where Are You!/Supernatural crossover episode in season 13, titled Scoobynatural.

In Scoobynatural, Dean and Sam are given a brand-new TV. When the TV starts to act odd, sending Sam, Dean, and Castiel into the Scooby-Doo, Where Are You! episode "A Night of Fright Is No Delight" where they team up with Daphne, Fred Jones, Scooby-Doo, Shaggy Rogers, and Velma Dinkley to solve a mystery. While Sam and Castiel want to use them to escape, Dean refuses to let them know the truth of their existence, as he wants to keep them "pure." Dean attempts to woo Daphne away from Fred, to no avail. After the Winchester Brothers reveal the existence of the supernatural, Daphne and the gang have a nervous breakdown, with the latter worrying she could possibly go to Hell since the afterlives exists. However, she and the gang are talked some sense into by the Brothers, who remind them of their accomplishments regarding past villains, although she and the gang, now introduced to the supernatural world, are at a high disadvantage as they are not mentally prepared for the truth. In the end, when Sam and Dean acknowledge this, the Winchesters and Castiel pretend the Phantom really was just Cosgood Creeps in a costume, and the Gang, satisfied, return to normal. Dean bids Daphne goodbye, gently "breaking it off" with her, although Daphne remains oblivious to this.*